SubtiBank SubtiBank
Version comparison:

2018-09-25 12:17:162025-05-22 12:35:08

title

yxjB

tlrB

description

unknown

23S rRNA (guanine-N(1)-)-methyltransferase, confers resistance against the macrolide antibiotic tylosin (produced by Streptomyces fradiae)

locus

BSU39010

BSU_39010

geneLength

846

849

function

unknown

resistance against tylosin

product

unknown

23S rRNA (guanine-N(1)-)-methyltransferase

ec

names

yxjB

tlrB

yxjB

outlinks

bsu

BSU39010

BSU_39010

Gene

Coordinates

4,004,288 → 4,005,136

4,004,288 4,005,136

Biological materials

Mutant

MGNA-B726 (yxjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1725 NBRP B. subtilis, Japan]

1A995 ( ''yxjB''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A995&Search=1A995 BGSC]

BKE39010 (Δ[[gene|tlrB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAAACACCCCAT, downstream forward: _UP4_TGAATGGTAGAGGAAAGCTC

BKK39010 (Δ[[gene|tlrB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAAACACCCCAT, downstream forward: _UP4_TGAATGGTAGAGGAAAGCTC

MGNA-B726 (yxjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1725 NBRP B. subtilis, Japan]

1A995 ( ''yxjB''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A995&Search=1A995 BGSC]

BKE39010 ([[gene|tlrB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAAACACCCCAT, downstream forward: _UP4_TGAATGGTAGAGGAAAGCTC

BKK39010 ([[gene|tlrB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAAACACCCCAT, downstream forward: _UP4_TGAATGGTAGAGGAAAGCTC

References

10746760, 27120414

10746760, 27120414, 14999102, 31719185

The protein

Catalyzed reaction/ biological activity

methylates G748 of 23S rRNA in the peptide exit tunnel [pubmed|31719185]

The protein

Protein family

[SW|Methyltransferase superfamily] (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|1P91] (from E. coli, 31% identity) [pubmed|14999102]